Improving Seed Viability and Germination – ATTRA – Sustainable Agriculture (2024)

By Andy Pressman, NCAT Northeast Regional Director

The Northeast is often known for the autumn season, a time when the landscape explodes in bright colors from the changing leaves. Leaf peepers travel from afar to witness this magical time of the year. For Northerners, early spring is another magical time of the year. What many refer to as the mud season, this is the time when the maple sugarhouses are boiling any remaining sap from late-season runs. Snow shoes are being hung up as thermal layers are being shed from bodies like a reptile shedding its skin. And while the pastures are still several weeks away from turning green, vegetable growers are hustling to start seeds in order to have transplants ready to go in to the ground once the soil has warmed up and dried out. Here at our farm in Southern New Hampshire, transplants are critical to our mixed vegetable operation and in having crops ready to harvest as early as possible.

Determining Seed Viability
Seed viability and good germination play critical roles in planning for a bountiful harvest. Many seed packets offer information on germination rates for the current year; however, seed viability decreases over time. Germination rates indicate how many seeds should be started in order to meet your targeted harvest yields, and they can save you time and money in having to replant if fewer seeds germinate than what you have expected.

Several factors can determine how long seeds are viable for, including how they stored from one season to the next. Temperature and humidity are critical components in storing seeds. At our farm in New Hampshire, we store our seeds in airtight containers in an unheated cellar. As a good rule of thumb, the temperature and humidity, when added together, should not be greater than 100. For example, with a temperature of 60°F, the humidity should be no more than 40%.

You can determine whether seeds are viable to plant with a simple test. Place 10 seeds on a damp paper towel and seal in a zip-lock plastic bag. The bag should be placed in a warm location, around 70 to 75°F. Check the seeds daily for germination. Most vegetable seeds sprout within three to seven days, with some exceptions, like the Umbelliferae family (carrots, parsley, parsnips, and others), which take longer. If there is even, or not sporadic, germination of 70% or higher, then the seeds are most likely good to use. I may plant a bit heavier if the rate is closer to 70%. Having five or fewer seeds germinate is a sign that the seeds are not very viable and you should not plant them. If six seeds germinate, well, it’s your call whether to plant, based on how much risk you are willing to take. The price of seed can be a factor in deciding whether or not to plant, and how much. As mentioned above, we look for even germination — germination around the same timeframe and not spread out. This year, we decided to build a low-tech, low-cost germination chamber. The goal is to increase germination rates by creating a space that provides uniform temperature and humidity for even seed germination. Commercial units can be very costly, so we set out to build one that was appropriate to our farm needs and our budget. We discovered several great DIY plans, including on the Broadfork Farm (Canada) website and in an article in the February 2020 issue of Growing for Market. Considering these design plans and others, we were able to utilize resources we had on the farm or that we could easily purchase (locally) while meeting our budget.

Improving Seed Viability and Germination – ATTRA – Sustainable Agriculture (1)

Inside the germination chamber. Notice the heater and water bucket on the bottom shelf.

Improving Seed Viability and Germination – ATTRA – Sustainable Agriculture (2)

Germination chamber with the front cover in place. Felix the cat keeps guard from up top.

Constructing a DIY Germination Chamber
The germination chamber is 48”W x 48”H x24”D and is constructed from 1” rigid foam insulation with reflective mylar film. The chamber was designed around the shelving unit and this size also makes for easy cutting of the insulation that come in 4’x8’ sheets. I doubled the top to 2” in order to make the chamber sturdier (and to handle the weight of our cat who likes to sleep on top). Only having 1” insulation on hand, I glued and bolted two 1” pieces together. The entire chamber is held together with heavy-duty construction tape.

Heat is supplied by an aquarium heater that we purchased, and we matched it to meet the size of the chamber. It sits in a rubber feed bucket that we top off with water daily. The heater has an external temperature setting, which is convenient as the box does not have to be opened in order to adjust the temperature. With this setup, we’ve been able to maintain an average temperature of around 73°F and 80% humidity.

The shelving unit has space for 11 seed trays with the heating unit taking up the space of one tray. We are able to stack trays three high, giving us the ability to have 33 trays in the chamber at one time. The shelves are made of particleboard, which I ripped into strips to allow for the heat to rise. This does require adding some type of support to the strips; I used scrap 1”x2” firing strips. The seed trays stay in the chamber until we see the first signs of germination. We do check the seed trays at least three times per day for germination. Because the seeds are started in complete darkness in the chamber, they can get leggy if the trays are not pulled out of the chamber immediately. Then the trays are placed under grow lights or in the greenhouse.

Improving Seed Viability and Germination – ATTRA – Sustainable Agriculture (3)

Once the first signs of germination are observed, the seed trays are taken out of the germination chamber and placed under lights or in the greenhouse.

Germination Chamber Results

We are pleased so far with the results of the germination chamber, especially with our plantings of greens. In addition to providing more uniform temperature and humidity, we are using less energy than the alternative use of heat mats, which also dry out the trays much quicker. We’ve observed that peppers are a bit slower to germinate in the chamber, as they tend to prefer slightly warmer temperatures than what we can provide with our setup. While we plan to further use the germination chamber, we may revert back to heat mats for peppers. Building a germination chamber makes economic sense, and costs can be reduced by using materials you already have.

Andy Pressman is an NCAT Agriculture Specialist and Director of NCAT’s Northeast Regional Office, located in Keene, NH. He and his family also operate Foggy Hill Farm, a small diversified farm and CSA. He can be reached at (479)587-3475 or andyp@ncat.org.

Estimated Budget
Rigid foam insulation (three 4’x8’ sheets) = $60

Heavy-duty construction tape = $9.00 roll

Shelving unit = $50

300-watt aquarium heater = $25

Related ATTRA Resources:

Potting Mixes for Certified Organic Production – Publication

Potting Mix – Video

Starting Seeds – Video

This blog is produced by the National Center for Appropriate Technology through the ATTRA Sustainable Agriculture program, under a cooperative agreement with USDA Rural Development. ATTRA.NCAT.ORG.

Photos: Andy Pressman, NCAT

Improving Seed Viability and Germination – ATTRA – Sustainable Agriculture (2024)

FAQs

How can we improve the viability of seeds? ›

Seeds need to be kept dry and cool. The most important factors in seed storage are seed moisture and storage temperature. The drier the seeds at time of storage, the longer they can be stored. Store in low humidity and low temperature, below 45°F (15 °C).

How does seed viability affect germination? ›

It was found that there is a high correlation between seed viability and germination capacity (r = 0.88) (figure 3). This indicates that viable seeds will guarantee a higher percentage of sprouted seeds, as an expression of the physiological quality of the seeds verified by the tetrazolium test.

Which is the most important factor for successful seed germination? ›

Thus, the most important factors required for seed germination is water, light, oxygen, and temperature. Soil: Soil is not a necessary requirement for seed germination. The soil just acts as a support material for the plants.

What are the 2 most important environmental conditions to get a seed to germinate? ›

All seeds need water, oxygen, and proper temperature in order to germinate. Some seeds require proper light also. Some germinate better in full light while others require darkness to germinate. When a seed is exposed to the proper conditions, water and oxygen are taken in through the seed coat.

How can seed germination be improved? ›

Providing consistent temperatures in the optimal range generally helps to ensure uniform germination. Using a thermostatically controlled seedling heat mat is an excellent way to provide the optimal temperature to germinating seeds.

How to make seeds viable? ›

Good germination occurs when water and oxygen are present at a favorable temperature. Good seed storage results when seeds are kept dry (below 8 percent moisture) and the temperature is kept low (below 40 degrees).

What are the 5 factors that affect seed germination? ›

Factors that Affect Seed Germination
  • Timing of planting.
  • Soil temperature.
  • Daylength.
  • Pretreatment of the seed, or lack thereof.
  • Growing conditions.

What are three factors that affect seed viability? ›

Moisture, temperature, and the proportion of oxygen are key environmental factors that affect seed deterioration and loss of viability.

What causes less viability in seeds? ›

Loss of viability is related to various seed properties, including color, weight, and membrane composition, which are often species or, in some cases, even variety specific [9]. Seed quality can be reduced in parental plants owing to adverse environmental conditions, premature germination [10] and pathogens [11].

How to make germination faster? ›

Here are some simple gardening tips for getting better, faster germination for all types of seeds.
  1. Pre-Soak Your Seeds Before Planting. ...
  2. Begin by Starting Your Seeds Indoors. ...
  3. Monitor Your Seed's Environment. ...
  4. Keep Them Well-Watered. ...
  5. Change Seed Sources.

What are the 4 critical factors for seed germination? ›

Temperature, moisture, air, and light conditions must be correct for seeds to germinate.

What are the five stages of seed germination? ›

The Seed Germination Process
  • Step 1: Imbibition: water fills the seed.
  • Step 2: The water activates enzymes that begin the plant's growth.
  • Step 3: The seed grows a root to access water underground.
  • Step 4: The seed grows shoots that grow towards the sun.
  • Step 5: The shoots grow leaves and begin photmorphogenesis.

What four needs to be met for a seed to germinate? ›

Light, water, oxygen, and temperature are essential conditions for seed germination. Its growth depends on many factors, including water, minerals, and nutrients. Water and oxygen are absorbed by the seed through its seed coat when it is exposed to the right conditions.

Why is seed germination important in agriculture? ›

For the majority of crops, seeds are the delivery system for transferring advanced genetics into the production field. In particular, rapid and synchronous seed germination and seedling growth are particularly important to agricultural output because they are essential for the establishment of seedlings in the field.

How can we improve seed quality? ›

Seed companies may also enhance seed quality at different steps of the seed production, by improving the methods of harvest but often by post-harvest treatments such as cleaning, sorting, coating, priming and controlling the storage conditions [1,11,12,13].

How to do seed viability? ›

The qualitative determination method with TTC involves cutting open the seed embryo and staining the embryo cells with TTC solution, allowing for quick qualitative assessment of seed viability based on whether the embryo is stained red or not.

How do you increase the shelf life of seeds? ›

Reducing humidity is the most-important factor in extending the life of seeds. In general, relative humidity below 60% is best for seed storage. Avoid storing seeds in sheds or garages where they may be exposed to high humidity in the summer. Store your seeds in a cool, dry location away from direct sunlight.

What are the factors affecting seed viability? ›

Relative humidity and temperature combination has to be provided for maintaining the viability. A combination of 25 per cent RH at 30oC temperature or less or a RH of about 45 per cent at 20oC or less will be ideal. The required RH can be achieved by making the room moisture proof and by using a dehumidifier.

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