How to Prevent Enzymatic Browning (2024)

How to Prevent Enzymatic Browning (1)

We’ve all seen it. We cut up an apple into slices and sometimes almost immediately the apple begins to turn brown. This is due to a chemical process which occurs in fruits and vegetables called enzymatic browning. It is caused by an enzyme called polyphenol oxidase which results in brown pigments when the cells from fruits or vegetables are exposed to oxygen. Bruising on fruit is caused by the same kind of reaction. When fruit is dropped, the cells at the place of impact are damaged and exposed to oxygen inside the fruit causing it to turn brown. Enzymatic browning can also be observed in seafood such as shrimp, crab, and spiny lobster. Enzymatic browning while not harmful can just make the affected fruit or vegetable less appealing to eat. Luckily there are ways to prevent or delay the browning. Preventing enzymatic browning on fruits. Brushing the surface of fruit with the juice from citrus fruits such as lemons, limes, grapefruits and oranges, you can prevent unsightly browning. Lemon juice is often the most commonly used for this technique.

Soaking in acidulated water: Placing fruit in water that has cider vinegar or lemon juice added to it can help prevent or delay enzymatic browning. How to Prevent Enzymatic Browning (2)The purpose of the lemon juice or cider vinegar is to reduce the pH on the surface of the fruit while the water will help reduce the amount of oxygen that either the fruit or vegetable is exposed to. Do not allow fruits to soak for too long. Fruits such as apples and pears should soak for no more than 15 minutes and bananas no longer than 5 minutes.

How to Prevent Enzymatic Browning (3)Cold water: Simply by placing peeled potatoes in cold water can prevent the browning. This only works with cold water. Warm or hot water will not prevent discoloring.

How to Prevent Enzymatic Browning (4)Blanching: This is the process of a short heat treatment to the fruit or vegetable in order to destroy or inactivate enzymes. Blanching softens the texture, brightens the color, and has little effect on the nutrient content and flavor of the fruit or vegetable. To blanch, boil some water in a pot; add the fruit or vegetable to the boiling water; and let sit for 30 seconds. Remove the product from the hot waterand place in an ice bath to cool down. The product is now blanched and ready to use.

Campus Tips:

  • Freshly cut fruit is available in most dining locations on campus with a Grab-n-Go fruit cup!
  • Want to precut an apple so it is ready toeat? After cutting, replace the pieces to form the original apple shape then wrap a rubber band around it to prevent it from browning! (The Rubber Band Trick | The Kitchn)

For more information on Enzymatic Browning and prevention visit:

Why Fruits and Vegetables Turn Brown

Enzymatic Browning

Ricardo Blanco, Student Assistant &Hospitality Dietitian Mindy Diller, MS, RDN, LD mindy.diller@ttu.edu

How to Prevent Enzymatic Browning (2024)

FAQs

How can we prevent enzymatic browning? ›

Thermal treatment, modified atmosphere storage, the prevention of oxygen exposure, low temperature, and irradiation are commonly used physical-based preservation systems to prevent enzymatic browning in fruit and vegetable [1].

Which of the following can prevent enzymatic browning? ›

Ascorbic acid, citric acid, sodium chlorite, and chelating agents are commonly used PPO inhibitors added to foods to prevent browning. Ascorbic acid, acting as a reducing agent and a competitive enzymatic inhibitor, reduces o-quinones back to o-diphenols preventing the formation of brown pigments.

How do we control enzymatic browning quizlet? ›

What are the physical methods for reducing enzymatic browning? Heat- pasteurisation - denaturation of the enzyme.

How can non enzymatic browning reactions be prevented? ›

Non-enzymatic browning and other quality deteriorating reactions during heat-processing of liquid foodstuffs are minimized by separately aseptically heat-processing the carbohydrate containing components and the nitrogeneous components and finally combining these processed materials at a temperature of at most 65° C.

What are the 4 ways to minimize enzymatic browning? ›

Physical methods to regulate enzymatic browning include thermal treatment, prevention of oxygen exposure, use of low temperature, and irradiation.

How to prevent enzymatic browning of apples? ›

To reduce browning in apples, keep the slices refrigerated to slow the reaction. You can also coat them with lemon or pineapple juice. The acids in these juices slow the reaction, and the antioxidants inhibit it.

Does salt prevent enzymatic browning? ›

Many experiments run by different research groups and individuals showed the similar results of having saltwater as the best treatment group to delay browning effects (Liang and Zhang; Pinola; Splawn). Moon et al. (2754) suggested that salt solution is a method for apple slices to avoid from contacting Oxygen.

Can enzymatic browning be reversed? ›

Enzymatic browning can be slowed or stopped by removing or changing the chemicals required for the browning reaction or changing the environment where the reaction is happening. Higher acidity, such as a pH below 4.0, inhibits the activity of the polyphenol oxidase (PPO) enzyme.

What affects enzymatic browning? ›

When fruit tissue is damaged because of heat, cold, age or mechanical stress, its cells break open and the phenolic compounds and the enzyme are released and mix with oxygen in the air. As a result the damaged tissue turns brown almost immediately.

How does vinegar prevent enzymatic browning? ›

Results. Lemon juice, Vinegar, clear soda will all prevent food from turning brown quickly. These liquids are acidic, so they will lower the pH of the food surface. Olive oil, will also prevent food from browning, but is less effective than the acids.

How do you decrease enzymatic browning when applied to cut fruit? ›

You can dip fruits in salt water or lemon water for 2-3 minutes. it prevents enzymatic browning.

How does milk prevent enzymatic browning? ›

... 39 Calcium caseinate and whey protein-based coatings on apple and potato slices reduced browning by acting as oxygen barriers, and reducing the gas transfer rates, the postharvest quality of coated apple and mango were also improved.

How do you control browning reactions? ›

Combining a chelating agent with an acid is an effective strategy for controlling enzymatic browning. Added acid limits enzyme activity by lowering the pH to levels below its optimum range. Ascorbic acid is a very effective inhibitor of enzymatic browning. Ascorbic acid lowers pH with minimal addition.

What are anti-browning agents? ›

Anti-browning agents are com- pounds that act primarily on either the enzyme or the intermediates of pigment formation. Their use in the food industry is constrained by issues such as toxicity, cost, and effects on taste, texture and color.

Does hot water prevent enzymatic browning? ›

Heat treatment alone is not sufficient to inhibit enzymatic browning in fresh-cut potato. Browning, in terms of color changes of peeled slices, was reduced only when HW dipping at 55 °C for 10 to 20 min was followed by storage at 20 °C for 1 d before processing.

Does water prevent enzymatic browning? ›

water Immersing the prepared fruits or vegetables in lemon juice and water (often referred to as acidulated water) slows down enzymic browning, but does not stop it altogether. The layer of acidic water helps limit the access of oxygen to the cell surfaces, but oxygen can still get through.

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